Dendritic and monocytic cells of cattle

One initial milestone was the identification of DC subsets (cDC1, cDC2, pDC) in blood of cattle and their transcriptional delineation from monocytes. Link to publication: Precise delineation and transcriptional characterization of bovine blood dendritic-cell and monocyte subsets
These experiments paved the way for characterizing transcriptional re-programming of bovine DC subsets upon in-vitro stimulation. Link to publication: Transcriptomic profling of bovine blood dendritic cells and monocytes following TLR stimulation
By combining bulk-and scRNA-seq, we revealed functional specialization and heterogeneity of bovine monocyte subsets in blood. Link to publication: Monocyte biology conserved across species: Functional insights from cattle
Our detailed scRNA-seq analysis of the mononuclear phagocyte system in bovine lymph node brought forward numerous hypotheses on differentiation trajectories and functional organization that likely represent features conserved across species. Link to publication: Single-cell transcriptomics reveals striking heterogeneity and functional organization of dendritic and monocytic cells in the bovine mesenteric lymph node
We continue to phenotype bovine dendritic cells and monocytes in lymphoid and nonlymphoid tissues (link to preprint: "Bovine dendritic-cell subsets in lymphoid and non-lymphoid tissues: work in progress"), and we engange in testing antibodies and recombinant proteins for phenotyping (link to preprint: "Expression of XCR1, CX3CR1, CSF1R and ADGRE1 on subsets of bovine blood dendritic cells and monocytes")
In a review article published in 2024, we provide an overview of phenotypic classifications in the bovine mononuclear phagocyte system. Link to review article: "Phenotype of bovine mononuclear phagocytes – An update"

Institute of Virology and Immunology

Group Talker

Dendritic and monocytic cells of cattle